Title | Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures. | ||
Author | de Vries, Michel; Pyrc, Krzysztof; Berkhout, Ron; Vermeulen-Oost, Wilma; Dijkman, Ronald; Jebbink, Maarten F; Bruisten, Sylvia; Berkhout, Ben; van der Hoek, Lia | ||
Journal | J Clin Microbiol | Publication Year/Month | 2008-Feb |
PMID | 18077635 | PMCID | PMC2238116 |
Affiliation | 1.Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center of University of Amsterdam, Amsterdam, The Netherlands. |
Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR.
StrainID | RV Species | Serotype | Length(nt) | Country | Year | Strain Name |
---|---|---|---|---|---|---|
UNS00782 | A | 102 | 7160 | Netherlands | AMS323 |