The purpose of this study was to determine the relationship between changes in heart rate variability (HRV), neuromuscular performance, and fatigue biomarkers in response to a resistance exercise bout. The root mean square of successive RR interval differences (RMSSD), neuromuscular performance - isometric handgrip (IHG), countermovement jump (CMJ), mean propulsive velocity (MPV) - metabolic stress (lactate [Lac]) and inflammation (interleukin-6 [IL-6]) were measured in 30 subjects who performed 6x10 back squat (BS), 3x10 bench press (BP), and 3x10 bent-over rows (BR) at 70% of 1-repetition maximum (1RM). The RMSSD, neuromuscular performance, and biomarkers were measured 10 min pre-exercise and 30 min post-exercise (Post(30)); HRV and Lac were also measured immediately post-exercise (Post(0)). Pre- versus post-exercise differences were evaluated using paired-samples t-tests. Pearson\'s correlations were used to determine the association between changes. With the exception of IL-6 (P=0.296) and MPV(BP) (P=0.678), LnRMSSD, neuromuscular performance, and metabolic stress were different post- compared to pre-exercise. We observed moderate associations between DeltaLnRMSSD Post(0) and DeltaLac Post(0) (r = -0.44) and DeltaLac Post(30) (r = -0.55), respectively. Practitioners should use multiple training load indicators to gain an accurate depiction of recovery.