| Title | Preferential recognition of the very low-density lipoprotein receptor ligand binding site by antibodies from phage display libraries. | ||
| Author | Pfistermueller, D M; Blaas, D; Hodits, R A | ||
| Journal | FEBS Lett | Publication Year/Month | 1996-Oct |
| PMID | 8906858 | PMCID | -N/A- |
| Affiliation | 1.Institute of Biochemistry, Medical Faculty, University of Vienna, Austria. | ||
Screening of a phage library displaying single chain fragments of the variable regions of human immunoglobulins (scFv) for binding to the ovarian chicken very low-density lipoprotein/vitellogenin receptor (OVR) led to the isolation of several antibody fragments with high affinity. As for the natural ligands of OVR, receptor binding of all antibody fragments is strictly Ca(2+)-dependent and is prevented by receptor-associated protein (RAP). Moreover, attachment of human rhinovirus serotype 2 (HRV2) to this receptor is inhibited by all scFvs. In contrast to conventional immunization, the in vitro selection method thus exclusively led to antibodies that attach to or close to the ligand binding site and thereby block the receptor-ligand interaction.