| Title | The 2A proteinase of human rhinovirus is a zinc containing enzyme. | ||
| Author | Sommergruber, W; Casari, G; Fessl, F; Seipelt, J; Skern, T | ||
| Journal | Virology | Publication Year/Month | 1994-Nov |
| PMID | 7941352 | PMCID | -N/A- |
| Affiliation | 1.BENDER+CO Ges. mbH, Department of Cell Biology, Vienna, Austria. | ||
The 2A proteinase of human rhinovirus (HRV) 2 is a cysteine proteinase which is not inhibited by metal chelating agents. However, total hydrolysis of highly purified HRV2 2A followed by atom emission spectroscopy demonstrated that HRV2 2A contains 1 mol of zinc per mole of enzyme. Furthermore, Zn depletion of the enzyme led to a loss of proteolytic activity which could subsequently be restored by Zn supplementation.