| Title | Localization of human rhinovirus replication in the upper respiratory tract by in situ hybridization. | ||
| Author | Arruda, E; Boyle, T R; Winther, B; Pevear, D C; Gwaltney, J M Jr; Hayden, F G | ||
| Journal | J Infect Dis | Publication Year/Month | 1995-May |
| PMID | 7751712 | PMCID | -N/A- |
| Affiliation | 1.Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908, USA. | ||
To localize the sites and determine the extent of human rhinovirus (HRV) replication in the upper respiratory tract, biopsies of nasal and nasopharyngeal epithelia were collected from 26 HRV- or 7 sham-inoculated volunteers on days 1, 3, and 5 and on days 12, 20, or 33 after inoculation and analyzed by in situ hybridization. HRV-infected cells were detected on at least 1 day in 22 of the 23 HRV-infected subjects and in 1 of the 7 sham-inoculated subjects who developed a cold and had nasal secretions positive for a picornavirus by polymerase chain reaction. Low numbers of in situ hybridization-positive ciliated cells were present in nasal biopsies. In the nasopharynx, most HRV-infected cells were ciliated, but infected nonciliated epithelial cells were also detected. Our results indicate that HRV replicates in a very small proportion of cells in the nasal epithelium and in both ciliated and nonciliated cells in the nasopharynx of experimentally infected humans.