| Title | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic. | ||
| Author | Ramos, Natalia; Panzera, Yanina; Frabasile, Sandra; Tomas, Gonzalo; Calleros, Lucia; Marandino, Ana; Goni, Natalia; Techera, Claudia; Grecco, Sofia; Fuques, Eddie; Coppola, Leticia; Ramas, Viviana; Morel, Maria Noelia; Mogdasy, Cristina; Chiparelli, Hector; Arbiza, Juan; Perez, Ruben; Delfraro, Adriana | ||
| Journal | Arch Virol | Publication Year/Month | 2023-Feb |
| PMID | 36786897 | PMCID | PMC9926447 |
| Affiliation + expend | 1.Seccion Virologia, Instituto de Biologia e Instituto de Quimica Biologica, Facultad de Ciencias, Universidad de la Republica, 4225, 11400, Igua, Montevideo, Uruguay. | ||
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.