OBJECTIVE: The aim of this clinical study was to determine whether the telemetric BioHarness 3.0(TM)chest strap (Zephyr Technology, Medtronic, Annapolis, USA), designed for use in humans and specialized for heart rate variability (HRV) analysis, could be used to accurately measure Heart Rate Variability (HRV) in pigs. METHODS: The R-wave intervals (RR-intervals) of the BioHarness 3.0(TM) electrocardiogram (ECG) were compared with those of the widely used telemetric ECG Televet((R))100 device (Engel Engineering GmbH, Heusenstamm, Germany). Measurements were performed under general anesthesia, to ensure continuous data collection due to the risk of detachment in adhesive electrodes of the Televet((R))100. The 2 devices were started simultaneously and measurements were taken 6 times in a row for 5 minutes, respectively. The data were collected from 5 male growing pigs. Following artifact correction resp. deletion 5321 RR paired data within a 3 digit range (ms) were analyzed statistically. RESULTS: The Lin Concordance-Correlation-Analysis after Lin (correlation coefficient 0.95), and the Bland-Altman-Analysis (RR distance differences + 0.3 ms) demonstrated a very good measurement compliance. CONCLUSION: This data suggests the BioHarness chest strap may be used for wireless HRV analysis in pigs as was shown in a follow up study in non-anesthetized pigs.