Objective: To investigate the molecular epidemiology of norovirus associated with pediatric acute gastroenteritis in Beijing under the Working Mechanism for Joint Prevention and Control of the Epidemic in 2020. Methods: This was a retrospective, repeated cross-sectional study. Fecal or vomit samples (1 213 cases) were collected from children visited the Capital Institute of Pediatrics Affiliated Children\'s Hospital for acute gastroenteritis from January 1 to December 31, 2020. First, real-time reverse PCR (RT-PCR) was used to screen the samples for norovirus, and then RdRp gene and capsid gene VP1 of norovirus-positive samples were amplified by conventional RT-PCR for genotyping based on the nucleotide sequence. The chi(2) test was used to compare the positive rates and genotypes of norovirus among different specimen types, genders of children, and different age groups. Results: Among the 1 213 samples were collected, 215 samples were positive for norovirus, with a positivity rate of 17.7% for the whole year. The peak of norovirus infection observed mainly in the cold seasons, as the positive rates were 28.6% (18/63), 26.2% (16/61), 22.8% (77/338) and 17.1% (89/520) in January, October, November and December, respectively. The positive rate of norovirus in fecal sample was significantly higher than that in vomit sample (chi(2) = 9.692, P<0.01). There was no significant difference between genders (chi(2)=0.041, P>0.05), but significant difference was found between age groups with the highest rate in the 6-48 months group (chi(2)=103.112, P<0.01). Three genogroups (G鈪? G鈪?and G鈪? of the circulating virus were detected by G-gene typing, and G鈪enogroup was predominant, accounting for 98.5% (196/199). Among the G鈪?positive samples, genotype G鈪?4 Sydney (55.1%, 108/196) was the most common, followed by G鈪?2 (29.6%, 58/196), while the G鈪?3 norovirus (10.2%, 20/196) which was common in previous years was not as much as before. Based on the P-type, G鈪?P16 was predominant (61.5%, 96/156), followed by GII.P31 (19.9%, 31/156). The dual genotyping revealed that G鈪?4 Sydney [P16] (36.4%, 56/154) and G鈪?2 [P16] (24.7%, 38/154) were predominant. Conclusion: The prevalence of norovirus in children in 2020 in Beijing is not much different from those of the previous years, but the genotypes composition has changed significantly, and there are multiple genotypes circulating simultaneously.