OBJECTIVES: To compare the acute effects of a single dose of beetroot juice (BJ) with different concentrations of nitrate (NO(3) (-)) on heart rate variability (HRV) in postmenopausal hypertensive women. METHODS: Thirteen hypertensive postmenopausal women (58.1 +/- 4.6 years of age and 27 +/- 4 kg/m(2) of BMI) completed the protocol that consisted of three visits with different beverage intakes in a randomized and crossover design. The three beverages were BJ with a high content of nitrate (high-NO(3) (-)), BJ with a low content of nitrate (low-NO(3) (-)), and an orange flavored non-caloric drink (OFD). Heart rate (HR) were evaluated during 20 min after sitting rest at 7:20 a.m. (baseline), after they drank one of the drinks, and remained at sitting rest for 120 min and then performed 40 min of aerobic exercise at 65-70% of the HR reserve on a treadmill. HR was recorded for 90 min after exercise for time, frequency, and non-linear domains of HRV index analysis. RESULTS: Two-way ANOVA showed that there were no interaction effects (time *sessions) in any of the HRV indexes after exercise in all three sessions. HRV indexes increased after exercise (p = <0.05) similarly in all three sessions when compared with the baseline time point. CONCLUSION: Therefore, a single dose of BJ, independent of NO(3) (-) content, does not change aerobic exercise-mediated responses in HRV indexes in hypertensive postmenopausal women.