BACKGROUND: There is controversial evidence regarding the effect of acute resistance exercise (ARE) on heart rate variability (HRV) parameters, which indicates the activities of the cardiac autonomic nervous system. The aim of this study was to perform a systematic review and meta-analysis of the literature on the effect of ARE on HRV parameters and identify its possible moderating factors. METHODS: The PubMed-Medline, Web of Science, SPORTDiscus, and Cochrane Library databases were searched. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) declaration was followed, and the methodological quality of the studies was evaluated. The level of significance was set at p </= 0.05. Twenty-six studies met the inclusion criteria. Main effect analyses between pre- and post-test interventions demonstrated an increase in normalized units low frequency (p < 0.001; standardized mean difference (SMD)鈥?鈥?.78; 95% confidence interval (95%CI): 0.46鈥?.11) and low frequency/high frequency ratio (p < 0.001; SMD鈥?鈥?.82; 95%CI: 0.64鈥?.99) and a decrease in standard deviation of the normal-to-normal (NN) interval (p < 0.001; SMD鈥?鈥?0.58; 95%CI: -0.85 to -0.30), root mean square of the successive differences (p < 0.001; SMD鈥?鈥?1.01; 95%CI: -1.29 to -0.74), and normalized units high frequency (p < 0.001; SMD: -1.08; 95%CI: -1.43 to -0.73) following ARE in healthy individuals range: 15 +/- 1 to 48 +/- 2 years; mean +/- SD). RESULTS: There were differences between the subgroups in the number of sets used in an exercise (p鈥?鈥?.05) for root mean square of the successive differences, as well as for exercise intensity (p鈥?鈥?.01) and rest between sets (p鈥?鈥?.05) for normalized units high frequency. Interestingly, there were differences between the subgroups in training volume for root mean square of the successive differences (p鈥?鈥?.01), normalized units high frequency (p鈥?鈥?.003) and normalized units low frequency (p鈥?鈥?.02). CONCLUSION: Overall, there was a withdrawal of cardiac parasympathetic and activation of cardiac sympathetic modulations following ARE, and these changes were greater with higher training volume approximately 30 min after ARE in healthy individuals. Furthermore, the number of sets, intensity, and rest between sets affected HRV parameters. However, gender, body mass index, and training status did not influence the changes in HRV parameters as a response to ARE.