| Title | NT*-HRV3CP: An optimized construct of human rhinovirus 14 3C protease for high-yield expression and fast affinity-tag cleavage. | ||
| Author | Abdelkader, Elwy H; Otting, Gottfried | ||
| Journal | J Biotechnol | Publication Year/Month | 2021-Jan |
| PMID | 33166527 | PMCID | -N/A- |
| Affiliation + expend | 1.ARC Centre of Excellence in Innovations in Peptide and Protein Science, Australian National University, Research School of Chemistry, Canberra, ACT 2601, Australia. | ||
The human rhinovirus 14 3C protease (HRV3C protease), in fusion with glutathione S-transferase also referred to as PreScission protease, is a cysteine protease of particular interest for affinity tag removal from fusion proteins due to its stringent recognition sequence specificity (LEVLFQ/GX) and superior activity at low temperature. Here we report the expression, purification and use of a fusion construct of HRV3C protease, NT*-HRV3CP, that affords high expression yield in E. coli (over 300mg/L cell culture), facile single-step purification, high solubility (>10mg/mL) and excellent storage properties. NT*-HRV3CP cleaves affinity tags at 4 degrees C in minutes, making it an attractive tool for the production of recombinant proteins for biotechnological, industrial and pharmaceutical applications.