| Title | Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma. | ||
| Author | Vanders, Rebecca L; Hsu, Alan; Gibson, Peter G; Murphy, Vanessa E; Wark, Peter A B | ||
| Journal | Respir Res | Publication Year/Month | 2019-Nov |
| PMID | 31747925 | PMCID | PMC6865028 |
| Affiliation + expend | 1.Priority Research Centre for Healthy Lungs, The University of Newcastle, Newcastle, NSW, Australia. rebecca.vanders@newcastle.edu.au. | ||
Respiratory virus-induced asthma exacerbations occur frequently during pregnancy and are associated with adverse outcomes for mother and child. Primary nasal epithelial cells (pNECs) provide a useful method to study immune responses in pregnancy. pNECs were obtained by nasal brushings from pregnant and non-pregnant women with and without asthma. pNECS were infected in vitro with major group Rhinovirus 43 (RV43) and seasonal influenza (H3N2). Following infection, pNECs showed measurable quantities of interferon (IFN)-lambda, IL-1beta, IL-8, IP-10 and MIP1-alpha. pNECs provide a safe and effective method for studying respiratory epithelial cell responses during pregnancy.