This study examined the effects of subject education and oximetry on discomfort sometimes associated with slow-paced breathing (dyspnea). This study was performed because some people report anxiety about getting sufficient oxygen while breathing slowly. Clinical experience suggested that reassuring subjects unaccustomed to slow-paced breathing that they are receiving enough oxygen may lead to greater comfort. The study had a sample size of 20 sequentially randomized healthy adults constituting two groups of 10 subjects. Both groups underwent 5 min of video-guided paced breathing at a rate of six breaths per minute. One group was able to view oximetry and hear an educational script, and the other received neither the educational script nor the viewable oximetry. Subjects answered a questionnaire about ease and adequacy of respiration as well as comfort. Analysis of the questionnaire showed that the group who received education about oximetry and viewed an oximeter during training felt significantly greater comfort during slow breathing than the conventional paced breathing group (p < 0.01). While further study is warranted, these preliminary findings suggest the potential need for this dyspneic effect to be taken into account in clinical practice as well as in research.