BACKGROUND: Meditation has been a key component of eastern spiritual practices. Heartfulness meditation is a unique heart based system with key practices like cleaning and meditation aided by yogic transmission. OBJECTIVE: To study the effects of Heartfulness cleaning and meditation (guided by Heartfulness trainer) on heart rate variability (HRV), blood pressure (BP) and heart rate (HR). METHODS: A total of 30 participants (21 males, 9 females; age range 19-70 years, M鈥?鈥?5.1鈥痽ears and SD鈥?鈥?2.7鈥痽ears) participated in the study. HRV, BP and HR were studied before, during and after the three stages of rest, cleaning and meditation. RESULTS: There was significant effect of cleaning and meditation on normalized unit of power in low-frequency band (LFnu) for the three conditions [F (2, 87)鈥?鈥?.98, p鈥?鈥?.01] with mean values for baseline being 70.82鈥?/-鈥?4.55, cleaning being 55.62鈥?/-鈥?5.06 and meditation being 55.17鈥?/-鈥?6.63. There was also a significant effect of cleaning and meditation on normalized unit of power in high-frequency band (HFnu) [F (2, 87)鈥?鈥?.31, p鈥?鈥?.01] with mean values for baseline being 30.86鈥?/-鈥?6.51, cleaning being 44.37鈥?/-鈥?5.06 and meditation being 44.83鈥?/-鈥?6.63. Significant effect of cleaning and meditation was also seen for LF/HF [F (2, 87)鈥?鈥?.98, p鈥?鈥?.01] with mean values for baseline being 3.45鈥?/-鈥?.40, cleaning being 1.63鈥?/-鈥?.30 and meditation being 1.82鈥?/-鈥?.19. CONCLUSION: Heartfulness cleaning and meditation had a positive effect on sympathovagal balance.