| Title | Comparative susceptibilities of strain MRC-5 human embryonic lung fibroblast cells and the Cooney strain of human fetal tonsil cells for isolation of rhinoviruses from clinical specimens. | ||
| Author | Geist, F C; Hayden, F G | ||
| Journal | J Clin Microbiol | Publication Year/Month | 1985-Sep |
| PMID | 2995440 | PMCID | PMC268434 |
The comparative susceptibilities for rhinovirus isolation were determined for strain MRC-5 human embryonic lung fibroblast and human fetal tonsil cells. Samples from nasopharyngeal and throat swab clinical specimens were inoculated onto duplicate monolayers of each cell type. Of 105 rhinovirus-positive specimens, 78 (74%) were positive in MRC-5 cells, and 102 (97%) were positive in fetal tonsil cells (P less than 0.001). For 75 specimens positive in both, the mean time to cytopathic effect development with standard deviation was 1 +/- 2 days shorter in fetal tonsil cells (P less than 0.01). The use of a second virus culture increased rhinovirus isolations by 10% compared with a single culture.