| Title | Innate Immune Response to Viral Infections in Primary Bronchial Epithelial Cells is Modified by the Atopic Status of Asthmatic Patients. | ||
| Author | Moskwa, Sylwia; Piotrowski, Wojciech; Marczak, Jerzy; Pawelczyk, Malgorzata; Lewandowska-Polak, Anna; Jarzebska, Marzanna; Brauncajs, Malgorzata; Globinska, Anna; Gorski, Pawel; Papadopoulos, Nikolaos G; Edwards, Michael R; Johnston, Sebastian L; Kowalski, Marek L | ||
| Journal | Allergy Asthma Immunol Res | Publication Year/Month | 2018-Mar |
| PMID | 29411555 | PMCID | PMC5809763 |
| Affiliation + expend | 1.Department of Immunology, Rheumatology and Allergy; Healthy Ageing Research Centre, Medical University of Lodz, Lodz, Poland. | ||
PURPOSE: In order to gain an insight into determinants of reported variability in immune responses to respiratory viruses in human bronchial epithelial cells (HBECs) from asthmatics, the responses of HBEC to viral infections were evaluated in HBECs from phenotypically heterogeneous groups of asthmatics and in healthy controls. METHODS: HBECs were obtained during bronchoscopy from 10 patients with asthma (6 atopic and 4 non-atopic) and from healthy controls (n=9) and grown as undifferentiated cultures. HBECs were infected with parainfluenza virus (PIV)-3 (MOI 0.1) and rhinovirus (RV)-1B (MOI 0.1), or treated with medium alone. The cell supernatants were harvested at 8, 24, and 48 hours. IFN-alpha, CXCL10 (IP-10), and RANTES (CCL5) were analyzed by using Cytometric Bead Array (CBA), and interferon (IFN)-beta and IFN-lambda1 by ELISA. Gene expression of IFNs, chemokines, and IFN-regulatory factors (IRF-3 and IRF-7) was determined by using quantitative PCR. RESULTS: PIV3 and RV1B infections increased IFN-lambda1 mRNA expression in HBECs from asthmatics and healthy controls to a similar extent, and virus-induced IFN-lambda1 expression correlated positively with IRF-7 expression. Following PIV3 infection, IP-10 protein release and mRNA expression were significantly higher in asthmatics compared to healthy controls (median 36.03-fold). No differences in the release or expression of RANTES, IFN-lambda1 protein and mRNA, or IFN-alpha and IFN-beta mRNA between asthmatics and healthy controls were observed. However, when asthmatics were divided according to their atopic status, HBECs from atopic asthmatics (n=6) generated significantly more IFN-lambda1 protein and demonstrated higher IFN-alpha, IFN-beta, and IRF-7 mRNA expressions in response to PIV3 compared to non-atopic asthmatics (n=4) and healthy controls (n=9). In response to RV1B infection, IFN-beta mRNA expression was lower (12.39-fold at 24 hours and 19.37-fold at 48 hours) in non-atopic asthmatics compared to atopic asthmatics. CONCLUSIONS: The immune response of HBECs to virus infections may not be deficient in asthmatics, but seems to be modified by atopic status.