| Title | Gene Knockdown in Human Rhinovirus 1B Using 2\'-OMe-modified siRNAs Results in the Reactivation of the Interferon Response. | ||
| Author | Xie, Guang Cheng; Zhang, Qing; Pang, Li Li; Li, Dan Di; Jin, Miao; Li, Hui Ying; Xu, Zi Qian; Kong, Xiang Yu; Wang, Hong; Lu, Shan; Duan, Zhao Jun | ||
| Journal | Biomed Environ Sci | Publication Year/Month | 2016-Feb |
| PMID | 27003171 | PMCID | -N/A- |
| Affiliation + expend | 1.Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China. | ||
The aim of this study was to investigate the knockdown efficiency of 2\'-O-methylated (2\'-OMe)-modified small interfering RNAs (siRNAs) on human rhinovirus 1B (HRV1B) replication and the interferon response. Thus, 24 2\'-OMe-modified siRNAs were designed to target HRV1B. The RNA levels of HRV1B, Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and interferons were determined in HRV1B-infected HeLa and BEAS-2B epithelial cells transfected with 2\'-OMe-modified siRNAs. The results revealed that all 2\'-OMe-modified siRNAs interfered with the replication of HRV1B in a cell-specific and transfection efficiency-dependent manner. Viral activation of Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and the interferon response was detected. In conclusion, the 2\'-OMe-modified siRNAs used in this study could interfere with HRV1B replication, possibly leading to the reactivation of the interferon response.