Heart rate variability (HRV) provides significant information about the health status of an individual. Optical heart rate monitoring is a comfortable alternative to ECG based heart rate monitoring. However, most available optical heart rate monitoring devices do not supply beat-to-beat detection accuracy required by proper HRV analysis. We evaluate the beat-to-beat detection accuracy of a recent wrist-worn optical heart rate monitoring device, PulseOn (PO). Ten subjects (8 male and 2 female; 35.9+/-10.3 years old) participated in the study. HRV was recorded with PO and Firstbeat Bodyguard 2 (BG2) device, which was used as an ECG based reference. HRV was recorded during sleep. As compared to BG2, PO detected on average 99.57% of the heartbeats (0.43% of beats missed) and had 0.72% extra beat detection rate, with 5.94 ms mean absolute error (MAE) in beat-to-beat intervals (RRI) as compared to the ECG based RRI BG2. Mean RMSSD difference between PO and BG2 derived HRV was 3.1 ms. Therefore, PO provides an accurate method for long term HRV monitoring during sleep.