| Title | The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus. | ||
| Author | Dyson, N; Buchkovich, K; Whyte, P; Harlow, E | ||
| Journal | Cell | Publication Year/Month | 1989-Jul |
| PMID | 2546678 | PMCID | -N/A- |
| Affiliation | 1.Cold Spring Harbor Laboratory, New York 11724. | ||
The association between the retinoblastoma protein (p105-RB) and either the large T antigen of SV40 or the E1A proteins of adenovirus is thought to be an important step in transformation by these viral oncogenes. E1A and large T antigen share a small region of amino acid homology that is necessary for high affinity binding with p105-RB. Mutations of this homology region were shown to reduce drastically the frequency of transformation mediated by the E1A or large T oncogenes. Previously, this small region in E1A was shown to be sufficient for interaction with a second cellular protein of 107,000 daltons (107K). Here we show that in human cells, the large T antigens of SV40 or JC virus also form complexes with 107K. Demonstration of complexes between 107K and the large T antigens of SV40 and JC virus suggests that these associations may represent another component of a common mechanism for transformation between adenoviruses and polyoma viruses.