| Title | FilmArray(R) respiratory panel performance in respiratory samples from neonatal care units. | ||
| Author | Piralla, Antonio; Lunghi, Giovanna; Percivalle, Elena; Vigano, Cristina; Nasta, Teresa; Pugni, Lorenza; Mosca, Fabio; Stronati, Mauro; Torresani, Erminio; Baldanti, Fausto | ||
| Journal | Diagn Microbiol Infect Dis | Publication Year/Month | 2014-Jun |
| PMID | 24666702 | PMCID | PMC7132758 |
| Affiliation + expend | 1.Molecular Virology Unit, Microbiology and Virology Department, Fondazione IRCCS Policlinico San Matteo, 27100, Pavia, Italy. | ||
FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15(R) detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.