From 2009 through to 2011, a long-run eco-medical monitoring was undertaken as a part of satellite project MARS-500. One of the objectives was field testing of innovative technologies of human functional state estimation. The monitoring protocol was a replica of that in the experiment with 520-day isolation. Heart Wizard Mars-500, a telemedical health estimation system based on heart rate variability analysis and pre-nosology diagnosis was trialed in the US and Canada. First and foremost the pre-nosology monitoring is aimed to estimate potential risks of maladaptation. Our results confirm diagnostic comprehensiveness of HRV analysis, particularly in long-run systematic health monitoring and when compared and contrasted with entries in the health and lifestyle questionnaire and functional test data. Heart Wizard Mars-500 is a simple and easy system for home use. Individual pre-nosology monitoring, initiation into own functional state and potential risk factors may inspire to change way of life and thus to improve health and life quality.