BACKGROUND: Heart rate variability (HRV) differs markedly by race, yet few studies have evaluated these relationships in women, and none have done so during sleep (sHRV). METHODS: We addressed these gaps by examining sHRV in women of African American, Chinese American, or European American origin or descent (mean [standard deviation] age = 51.2 [2.2] years). RESULTS: HRV during Stage 2 non-rapid eye movement (NREM) and rapid eye movement (REM) sleep differed significantly by race after adjusting for possible confounders. Normalized high-frequency HRV was significantly lower in European American compared with African American and Chinese American participants (European American NREM = 0.35 [0.01], REM = 0.23 [0.01]; African American NREM = 0.43 [0.02], REM = 0.29 [0.02]; Chinese American NREM = 0.47 [0.03], REM = 0.33 [0.02]; p values <.001). European Americans also exhibited higher low-to-high-frequency HRV ratios during sleep compared with African American and Chinese American women (European American NREM = 2.42 [1.07], REM = 5.05 [1.07]; African American NREM = 1.69 [1.09], REM = 3.51 [1.09]; Chinese American NREM = 1.35 [1.07], REM = 2.88 [1.13]; p values <.001). CONCLUSIONS: Race was robustly related to sHRV. Compared with women of African American or Chinese American origin or descent, European American women exhibited decreased vagally mediated control of the heart during sleep. Prospective data are needed to evaluate whether sHRV, including race differences, predicts cardiovascular disease.