| Title | Rhinovirus infection causes steroid resistance in airway epithelium through nuclear factor kappaB and c-Jun N-terminal kinase activation. | ||
| Author | Papi, Alberto; Contoli, Marco; Adcock, Ian M; Bellettato, Cinzia; Padovani, Anna; Casolari, Paolo; Stanciu, Luminita A; Barnes, Peter J; Johnston, Sebastian L; Ito, Kazuhiro; Caramori, Gaetano | ||
| Journal | J Allergy Clin Immunol | Publication Year/Month | 2013-Nov |
| PMID | 23871663 | PMCID | -N/A- |
| Affiliation | 1.Section of Respiratory Diseases, Department of Medical Sciences, University of Ferrara, Ferrara, Italy. Electronic address: ppa@unife.it. | ||
BACKGROUND: Although inhaled glucocorticoids are the mainstays of asthma treatment, they are poorly effective at treating and preventing virus-induced asthma exacerbations. The major viruses precipitating asthma exacerbations are rhinoviruses. OBJECTIVE: We sought to evaluate whether rhinovirus infection interferes with the mechanisms of action of glucocorticoids. METHODS: Cultured primary human bronchial or transformed (A549) respiratory epithelial cells were infected with rhinovirus 16 (RV-16) before dexamethasone exposure. Glucocorticoid receptor (GR) alpha nuclear translocation, glucocorticoid response element (GRE) binding, and transactivation/transrepression functional readouts were evaluated by using immunocytochemistry, Western blotting, DNA binding assays, real-time quantitative PCR, coimmunoprecipitation, and ELISA techniques. Specific inhibitors of c-Jun N-terminal kinase (JNK) and of IkappaB kinase (IKK) were used to investigate the involvement of intracellular signaling pathways. RESULTS: RV-16 infection impaired dexamethasone-dependent (1) inhibition of IL-1beta-induced CXCL8 release, (2) induction of mitogen-activated protein kinase phosphatase 1 gene expression, and (3) binding of GR to GREs in airway epithelial cells. This was associated with impaired GRalpha nuclear translocation, as assessed by means of both immunochemistry (54.0% +/- 6.8% vs 24.7% +/- 3.8% GR-positive nuclei after 10 nmol/L dexamethasone treatment in sham- or RV-16-infected cells, respectively; P < .01) and Western blotting. RV-16 infection induced nuclear factor kappaB activation and GRalpha phosphorylation, which were prevented by inhibitors of IKK2 and JNK, respectively. In rhinovirus-infected cells the combination of JNK and IKK2 inhibitors totally restored dexamethasone suppression of CXCL8 release, induction of mitogen-activated protein kinase phosphatase 1 gene expression, and GRalpha nuclear translocation. CONCLUSION: RV-16 infection of human airway epithelium induces glucocorticoid resistance. Inhibition of RV-16-induced JNK and nuclear factor kappaB activation fully reversed rhinovirus impairment of both GRalpha nuclear translocation and the transactivation/transrepression activities of glucocorticoids.