Title Role of anti-beta1 adrenergic antibodies from patients with periodontitis in cardiac dysfunction.
Author Segovia, Marcela; Ganzinelli, Sabrina; Reina, Silvia; Borda, Enri; Sterin-Borda, Leonor
Journal J Oral Pathol Med Publication Year/Month 2012-Mar
PMID 21958237 PMCID -N/A-
Affiliation 1.Pharmacology Unit, School of Dentistry, Buenos Aires University, Buenos Aires, Argentina.

BACKGROUND: The presence of serum autoantibodies against beta(1) adrenoreceptors (beta(1)-ARs) in human gingival fibroblast from patients with periodontitis inhibits primary cell-specific growth and induces over-expression of pro-inflammatory mediators. Serum beta(1)-AR autoantibodies from patients with periodontitis react with myocardium and modify cardiac contractility. The relationship between the presence of serum beta(1)-AR autoantibodies and alterations in heart rate variability (HRV) was also studied. METHODS: An enzyme-linked immunosorbent assay (ELISA) using cardiac and gingival fibroblast membranes or synthetic peptides corresponding to the second extracellular loop of human beta(1)-AR was used to detect serum autoantibodies. The HRV was assessed from RR interval files generated from 22:00 to 08:00 hours. The autoantibody effects on contractility were measured on spontaneous rat isolated atria. RESULTS: Circulating autoantibodies from 36 patients with periodontitis and 20 healthy individuals (controls) interacted with fibroblasts, the cardiac surface, and beta(1)-AR synthetic peptides. The distributions of serum antibodies against gingival and myocardium membranes and beta(1)-AR synthetic peptide were 88.8%, 77.7%, and 92.8%, respectively. Moreover, 88.5% of patients with periodontitis whose sera were positive against beta(1)-AR synthetic peptide had decreased HRV. The corresponding affinity-purified anti-beta(1)-AR peptide IgG displayed partial agonist-like activity modifying the isolated atria contractility. CONCLUSION: This manuscript describes that patients with periodontitis showed increased levels of serum IgG with reactive activity against beta(1)-AR. Those patients demonstrated decrease in heart rate, and IgG derived from their sera induced aberrant contractility of heart atrium. We propose that periodontitis increases the risk of cardiovascular diseases, although it increases anti-beta(1)-AR autoantibody that alters myocardial contractility.

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