| Title | Determination of the kinetic on- and off-rate of single virus-cell interactions. | ||
| Author | Rankl, Christian; Wildling, Linda; Neundlinger, Isabel; Kienberger, Ferry; Gruber, Hermann; Blaas, Dieter; Hinterdorfer, Peter | ||
| Journal | Methods Mol Biol | Publication Year/Month | 2011 |
| PMID | 21660729 | PMCID | -N/A- |
| Affiliation | 1.Institute for Biophysics, University of Linz, Linz, Austria. | ||
Human rhinoviruses are the causative agents of the common cold. The serotypes belonging to the minor receptor group attach to members of the low-density lipoprotein receptor family and enter the host cell via receptor-mediated endocytosis. Receptor binding, the very first step in infection, was characterized by force spectroscopy measurements at the single molecule level. We demonstrate how kinetic on- and off-rate constants can be derived from such experiments carried out with the atomic force microscope.