| Title | Usefulness of published PCR primers in detecting human rhinovirus infection. | ||
| Author | Faux, Cassandra E; Arden, Katherine E; Lambert, Stephen B; Nissen, Michael D; Nolan, Terry M; Chang, Anne B; Sloots, Theo P; Mackay, Ian M | ||
| Journal | Emerg Infect Dis | Publication Year/Month | 2011-Feb |
| PMID | 21291610 | PMCID | PMC3204776 |
| Affiliation | 1.The University of Queensland, Brisbane, Queensland, Australia. | ||
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.