Productivity bears a close relationship to the indoor environmental quality (IEQ), but how to evaluate office worker\'s productivity remains to be a challenge for ergonomists. In this study, the effect of indoor air temperature (17 degrees C, 21 degrees C, and 28 degrees C) on productivity was investigated with 21 volunteered participants in the laboratory experiment. Participants performed computerized neurobehavioral tests during exposure in the lab; their physiological parameters including heart rate variation (HRV) and electroencephalograph (EEG) were also measured. Several subjective rating scales were used to tap participant\'s emotion, well-being, motivation and the workload imposed by tasks. It was found that the warm discomfort negatively affected participants\' well-being and increased the ratio of low frequency (LF) to high frequency (HF) of HRV. In the moderately uncomfortable environment, the workload imposed by tasks increased and participants had to exert more effort to maintain their performance and they also had lower motivation to do work. The results indicate that thermal discomfort caused by high or low air temperature had negative influence on office workers\' productivity and the subjective rating scales were useful supplements of neurobehavioral performance measures when evaluating the effects of IEQ on productivity.