| Title | Simultaneous detection of different respiratory virus by a multiplex reverse transcription polymerase chain reaction combined with flow-through reverse dot blotting assay. | ||
| Author | Li, Pei-qiong; Yang, Zi-feng; Chen, Jing-xian; Muller, Claude P; Zhang, Jun; Wang, Dan-fen; Zhang, Ren; He, Yun-shao | ||
| Journal | Diagn Microbiol Infect Dis | Publication Year/Month | 2008-Sep |
| PMID | 18639996 | PMCID | -N/A- |
| Affiliation | 1.Department of Cell Biology and Genetics, School of Basic Science, Guangzhou Medical College, Guangzhou, Guangdong, China. | ||
Cell culture and immunofluorescence (IF) assays have been traditionally used for the laboratory diagnosis of respiratory viral infections, but these assays have a low sensitivity and are time consuming. We developed a multiplex reverse transcription polymerase chain reaction combined with flow-through reverse dot blotting (mRT-PCR-FT-RDB) assay for the simultaneous detection of influenza virus type A including H5 subtype and H9 subtype, influenza virus type B, parainfluenza virus types 1 and 3, respiratory syncytial virus, human rhinovirus, and human coxsackievirus. In comparison with viral culture and IF assay as the gold standard method, the mRT-PCR-FT-RDB assay gave a sensitivity and a specificity of 100% and 98%. The high sensitivity and specificity, the rapid result turnaround time, and the reduced expense of the mRT-PCR-FT-RDB assay compared with viral culture and IF assay suggest that this assay would be a significant improvement over traditional ones for the detection of respiratory viruses in a clinical laboratory.