| Title | Localization of rhinovirus replication in vitro with in situ hybridization. | ||
| Author | de Arruda, E 3rd; Mifflin, T E; Gwaltney, J M Jr; Winther, B; Hayden, F G | ||
| Journal | J Med Virol | Publication Year/Month | 1991-May |
| PMID | 1653307 | PMCID | PMC7166441 |
| Affiliation | 1.Department of Internal Medicine, University of Virginia Health Sciences Center, Charlottesville 22908. | ||
To facilitate understanding of human rhinovirus (HRV) pathogenesis, methods were developed for detection of HRV infection in vitro using in situ hybridization (ISH). HRV-14 RNA probes and oligonucleotide probes representing conserved sequences in the 5\'-non-translated region were labeled with 35S and used to detect infected HeLa or WI-38 strain human embryonic lung cells in cytological preparations. ISH was shown to be specific for detection of HRV on a single-cell basis. Subsequently, in human nasal polyps infected in vitro, both oligonucleotide- and ribo-probes produced a strong signal in association with ciliated epithelial cells. In human adenoids infected in vitro, a signal was observed in non-ciliated epithelial cells. This study shows that HRV replicates in ciliated cells in the epithelium of human nasal polyps infected in vitro, and the presence of viral RNA in non-ciliated cells of the human adenoid infected in vitro suggests that other cell types may also support rhinovirus replication.