| Title | The proton pump inhibitor lansoprazole inhibits rhinovirus infection in cultured human tracheal epithelial cells. | ||
| Author | Sasaki, Takahiko; Yamaya, Mutsuo; Yasuda, Hiroyasu; Inoue, Daisuke; Yamada, Mitsuhiro; Kubo, Hiroshi; Nishimura, Hidekazu; Sasaki, Hidetada | ||
| Journal | Eur J Pharmacol | Publication Year/Month | 2005-Feb |
| PMID | 15733557 | PMCID | -N/A- |
| Affiliation | 1.Department of Geriatric and Respiratory Medicine, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan. | ||
To examine the effects of lansoprazole, a proton pump inhibitor, on rhinovirus infection in airways, human tracheal epithelial cells were infected with a major subgroup of rhinoviruses, type 14 rhinovirus. Rhinovirus increased the mRNA expression of intercellular adhesion molecule-1 (ICAM-1) in the cells, the major rhinovirus receptor, and the content of the soluble form of ICAM-1 (sICAM-1) and cytokines in supernatants. Lansoprazole reduced supernatant titers and RNA of rhinovirus, the susceptibility to rhinovirus infection, the ICAM-1 mRNA production, the number and fluorescence intensity of acidic endosomes in the cells, and supernatants sICAM-1 and cytokine concentrations including interleukin-1beta. Antibody to interleukin-1beta reduced baseline and rhinovirus-induced ICAM-1 production. These results suggest that lansoprazole inhibits rhinovirus infection by reducing ICAM-1 via partly endogenous production of interleukin-1beta, and by blocking the rhinovirus RNA entry into the endosomes. Lansoprazole may modulate airway inflammation by reducing the production of cytokines and ICAM-1 in rhinovirus infection.