The purpose of this study was to clarify the effect of visual and auditory stimulation on emotions and autonomic nervous systems. Four kinds of video films, which themes were humor, discomfort, fear or gentleness were presented to 14 subjects. Spectral analysis of heart rate variability (HRV) was observed as the autonomic nerve responses. The four kinds of video films caused different emotional reactions (the difference in the reaction of the autonomic nervous systems to each video film are shown). It was confirmed that the autonomic nerve responses differed in accordance to how the video film was recognized by the subjects. When television and videos are utilized for nursing care, it is important to offer them to patients, taking into consideration their character and preference. In addition, individual care should be carried out by observing the emotional reactions of patients.