| Title | Changes in rhinovirus protein 2C allow efficient replication in mouse cells. | ||
| Author | Harris, Julie R; Racaniello, Vincent R | ||
| Journal | J Virol | Publication Year/Month | 2003-Apr |
| PMID | 12663784 | PMCID | PMC152148 |
| Affiliation | 1.Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA. | ||
Rhinovirus type 16 was found to replicate in mouse L cells that express the viral receptor, human intercellular adhesion molecule 1 (ICAM-1). However, infection of these cells at a low multiplicity of infection leads to no discernible cytopathic effect, and low virus titers are produced. A variant virus, 16/L, was isolated after alternate passage of rhinovirus 16 between HeLa and ICAM-1 L cells. Infection of mouse cells with 16/L leads to higher virus titers, increased production of RNA, and total cytopathic effect. Three amino acid changes were identified in the P2 region of virus 16/L, and the adaptation phenotype mapped to two changes in protein 2C. The characterization of a rhinovirus host range mutant will facilitate the investigation of cellular proteins required for efficient viral growth and the development of a murine model for rhinovirus infection.