Possible effects of vestibular stimulation autonomic nerve function in pilots were studied by observing changes of Heart Rate Variability (HRV). Time domain and frequency domain analyses demonstrated that the primarily averages, standard deviation of the HRV averages(SD), least or greatest value, deviation of greatest minus least value, interrelated averages, square root of the mean of the sum of squares of differences between adjacent R-R intervals (RMSSD), total power(TP), low frequency(LF), high frequency(HF), LF/TP, HF/TP, and LF/HF all had significant increase or decrease (P <0.05 - <0.01) and the degrees of changes were related to the intensity of the stimulations.