| Title | S-nitrosothiols as novel, reversible inhibitors of human rhinovirus 3C protease. | ||
| Author | Xian, M; Wang, Q M; Chen, X; Wang, K; Wang, P G | ||
| Journal | Bioorg Med Chem Lett | Publication Year/Month | 2000-Sep |
| PMID | 10999479 | PMCID | -N/A- |
| Affiliation | 1.Department of Chemistry, Wayne State University, Detroit, MI 48202, USA. | ||
Human rhinovirus (HRV) 3C protease was inactivated by a series of S-nitrosothiols. These compounds exhibited different inhibitory activities in a time- and concentration-dependent manner with second-order rate constants (kinact/K(I)) ranging from 131 to 5360 M(-1) min(-1). The inactive enzyme could be re-activated by DTT, GSH and ascorbate, which indicated the inactivation mechanism was through an S-transnitrosylation process.