| Title | Identification of human rotavirus strains with the P[14] genotype by PCR. | ||
| Author | Arista, S; Vizzi, E; Alaimo, C; Palermo, D; Cascio, A | ||
| Journal | J Clin Microbiol | Publication Year/Month | 1999-Aug |
| PMID | 10405427 | PMCID | PMC85321 |
| Affiliation | 1.Dipartimento di Igiene e Microbiologia, Universita di Palermo," Palermo, Italy. | ||
A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].