| Title | Position-dependent processing of peptides presented on the surface of cowpea mosaic virus. | ||
| Author | Taylor, K M; Porta, C; Lin, T; Johnson, J E; Barker, P J; Lomonossoff, G P | ||
| Journal | Biol Chem | Publication Year/Month | 1999-Mar |
| PMID | 10223342 | PMCID | -N/A- |
| Affiliation | 1.Dept. of Virus Research, John Innes Centre, Norwich, UK. | ||
The plant virus cowpea mosaic virus (CPMV) has been developed as an epitope-presentation system. Numerous epitopes have been expressed in the betaB-betaC loop of the CPMV small coat protein, all of which undergo a cleavage reaction between their two carboxy-terminal residues. Although many peptides presented in this manner give an authentic immune response, this was not the case for the NIm-1A epitope from human rhinovirus-14. Crystallography revealed significant differences between the structure of NIm-1A on CPMV compared with its native configuration. The 3D structure of C PMV expressing NIm-1A was used to design alterations to the context of the NIm-1A graft.